Coding

Part:BBa_K4271004:Design

Designed by: Yi-Chen Lin   Group: iGEM22_Wego_Taipei   (2022-09-09)


pNP Mut 1-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pNP Mut 1-1 protein is derived from the pobR protein. The pobR protein, under normal circumstances, has higher affinity to the chemical 4-hydroxybenzoate (4HB). For experimental purposes, this protein is engineered to have greater affinity to pNP. The new protein, pNP Mut 1-1, enhances GFP expression (Jha et al. 8495).


Source

BL21

References